+
+There are four factory presets for the accelerating voltage (5 kV, 10 kV, 20 kV, 30 kV), one
+for each HV index. The user does not need to make any further adjustments by switching
+among them and using magnification up to 4000x.
+
+
+
+Click on the PUMP button in the Vacuum panel to start the pumping procedure
+(Figure 2). It usually takes around 3 minutes to reach vacuum ready - status which
+means that the microscope is ready to use. If there is a need to exchange the
+specimen, follow the instructions in chapter 8.2.
+
+
+
+
+
+20240310-112336_screenshot.png
+
+
+
+In the SEM Detectors & Mixer panel select the appropriate detector from the list box
+(Figure 3). We recommend using the SE or BSE detector. When the BSE detector is
+used, make sure that the detector is not retracted! See chapter 6 for detailed infor-
+mation
+
+
+
+
+20240310-112456_screenshot.png
+
+
+
+
+
+20240310-112616_screenshot.png
+
+
+
+
+- Select the accelerating voltage (30 kV recommended) using the combo box in the
+
+
+Electron Beam panel (Figure 5).
+
+
+- Clicking on the HV button in the Electron Beam panel turns the high voltage on and
+
+
+starts the heating of the tungsten filament (see Figure 5).
+
+
+- Right-click in the SEM Scanning window to open the menu and select the Minimum
+
+
+Magnification function (Figure 6)
+
+
+
+
+20240310-112645_screenshot.png
+
+
+
+
+20240310-112723_screenshot.png
+
+
+
+
+7 Select RESOLUTION mode (click on the Scan Mode function in the Info Panel (see
+Figure 10) and select RESOLUTION or use the Continual Wide Field option – switches
+automatically between WIDE FIELD and RESOLUTION mode and vice versa when
+increasing or decreasing magnification)
+
+
+
+Focus the image by clicking on the WD icon in the Toolbar and turning the
+Trackball from left to right (or vice versa). Alternatively use the Auto WD function for
+focusing (see Figure 6). Double-clicking (left mouse button) in the SEM Scanning
+window opens the Focus window. To remove the Focus window double-click
+anywhere in the SEM Scanning window.
+
+
+
+
+To select beam intensity (BI 10 recommended), first left-click on the BI icon
+on the Toolbar and then use the arrows in the Pad panel (Figure 8).
+
+
+
+
+
+20240310-113043_screenshot.png
+
+
+
+- To select the sample position in the Stage Control panel, click on the appropriate
+
+
+number button on the carousel (Figure 9) or use the manual knobs in the case of the
+SB microscope type.
+
+
+- Placing the cursor over the SEM Scanning window and clicking the mouse wheel
+
+
+moves that area on the stage into the centre of the image. See chapter 7.2 for other
+mouse actions.
+
+
+- To magnify the image click on the Magnification icon on the Toolbar and turn
+
+
+the Trackball from left to right.
+
+
+- Once the area of interest is magnified and focused as desired, right-click on the
+
+
+Speed icon on the Toolbar and select the appropriate scanning speed.
+
+
+- Clicking on the Acquire button in the Info Panel (Figure 10) or on the icon
+
+
+on the Toolbar saves the image. Fill in the note, sign and description field
+if necessary. Choose a folder in which to store the image. To change the parameters
+of the image use the Image Parameters function in the main SEM menu
+
+
+
+
+20240310-113153_screenshot.png
+
+
+
+
+20240310-113251_screenshot.png
+
+
+
+- Clicking on the icon opens the dialogue for saving the actual adjustment of the
+
+
+microscope. It is possible to restore the saved adjustment of the microscope later.
+
+